CTCF functions as an insulator for somatic genes and a chromatin remodeler for pluripotency genes during reprogramming

CTCF functions as an insulator for somatic genes and a chromatin remodeler for pluripotency genes during reprogramming

Blog Article

Summary: CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes.Here, Full Panel Headboard we show that Ctcf expression is progressively increased during induced pluripotency.In this process, CTCF first functions as a chromatin insulator responsible for direct silencing of the somatic gene expression program and, interestingly, elevated Ctcf expression next ensures chromatin accessibility and contributes to increased EP interactions for a fraction of pluripotency-associated genes.

Therefore, CTCF functions in a context-specific manner to modulate the 3D genome to enable cellular reprogramming.We further discover that these context-specific CTCF functions also enlist SMARCA5, an imitation switch (ISWI) chromatin remodeler, together rewiring Barstool the epigenome to facilitate cell-fate switch.These findings reveal the dual functions of CTCF in conjunction with a key chromatin remodeler to drive reprogramming toward pluripotency.